Waxay ka ciyaartaa door muhiim ah hababka nolosha, fosfooraska Tyrr, Ser, Thr, oo ku yaal peptides.Fosforylated amino acid waxaa caadi ahaan loo isticmaalaa soo saarista phosphoric acid peptide, oo hadda loo isticmaalo hal asiidh fosfooryl fenyl ah.HBTU/phosphorylated amino acids ayaa sida caadiga ah loo isticmaalaa in lagu xidho fosfooraska amino acids HOBt/DIEA, laakiin fosfooraska acid peptide ayaa dooranaya cilladaha habkan, gaar ahaan peptides peptides dheer ama fosfate amino acid, isku xirka waxtarka ayaa hooseeya, hooseeya. daahirsanaanta alaabta kama dambaysta ah.Fosfopeptide-kan, waxaanu u tixgelinay habka fosfooraska ka dambeeya ee xulashada ah.Habka jiilku waa ka saarista xulashada ee kooxaha ilaalinta silsiladda amino acid kuwaas oo u baahan in lagu calaamadiyo ka dib isku-darka peptide.Dhanka Tyr, Thr wuxuu si toos ah uga falcelin karaa iyadoo la isticmaalayo asiidhyada amino-ka ee leh silsilado dhinac ah oo aan la ilaalin.-Ser (trt), tiro ahaan laga saaray 1% TFA/DCM deegaanka.
Phosphorylation, oo leh labanlaab benzene fosfooraska fosphoramide iyo afar nitrogen ayaa la abuuraa iyo fosphoramide tetrazolium dhexdhexaadiye firfircoon, oo ku xiran hydroxy, ka dibna hoos yimaada acid peroxide oxidation fosfooryl, falcelin dhamaystiran.
Waqtigan xaadirka ah, habka wax ka beddelka peptide fosfooraska waxaa jira laba nooc oo waaweyn:
(1) Soo bandhigida tooska ah ee asiidhyada amino fosforylated ee dhexdhexaadka ah ee dammaanad-qaadka ah ee isku xigxiga polypeptide;
(2) ka dib xabagta uu ka soo saaray isku xigxiga peptide, ilaa Ser, Tyr, ama Thr fosforyaalka ee silsiladda hydroxyl.
1) waxay dhexdhexaadin doontaa dammaanad qaadka fosfooraska amino acids si toos ah u soo bandhigaya taxanaha peptide:
Taasi waa, asiidhyada amino ee u baahan fosforyaalka ayaa la sii dayn doonaa oo si habboon loo ilaalin doonaa ka hor iyadoo la isticmaalayo (Thr, Ser ama Tyr), ka dibna fosforyaalka waxaa lagu soo koobayaa meesha xulashada tusaha polypeptide ee ku salaysan habka caadiga ah ee jiilka SPPS. .Habkani waa sahlan tahay in la shaqeeyo, wuxuu noqday habka ugu muhiimsan ee shirkadaha peptide ee wax ka beddelka fosfooraska.
2) ka dib xabagta ka soo baxday isku xigxiga peptide, ilaa Ser hydroxy fosforyaalka, Tyr, ama Thr silsiladaha dhinaca:
Qurxinta fosforyaalka iyadoo la adeegsanayo fosforyaalka hal foorarsiga habka polypeptide, sababtoo ah caqabada adag ee ay abuuraan kooxo waaweyn oo qurxinta silsiladda dhinaca, way adag tahay fosforyaalka amino acids si ay u yareeyaan silsiladda peptide.Mustaqbalka, hordhaca asiidhyada amino-ka ayaa noqon doona mid aad u adag, gaar ahaan qurxinta ka kooban goobo fosforyaal badan, jiilku wuxuu noqon doonaa mid aad u adag, wax soo saarka ugu dambeeya waa mid adag, adag tahay in la kala saaro, iyo wax-soo-saarka ayaa aad u hooseeya.
Sidaa darteed, marka goobo badan oo silsiladda peptide ah lagu daro fosfooraska, mid ayaa dooran kara in lagu soo saaro isku xigxiga peptide ee resinka ka dibna fosforyaal ku dheji kooxda hydroxyl ee Ser, Tyr, ama Thr: jiilkeeda inta badan waxay ku lug leedahay ka saarista xulashada Kooxda ilaalinta silsiladda dhinac ee u baahan aqoonsiga amino acids ka dib isku-darka peptide.Marka loo eego Tyr, Thr waxay si toos ah u isticmaali kartaa silsiladaha dhinaca amino acids ma ilaalinayaan jawaabta.
Saldhig ilaalinta silsiladda dhinaca 1% deegaanka TFA/DCM ayaa tiro ahaan laga saari karaa.Marka habkan la doorto, fosphoramidite tetrazolium firfircoon dhexdhexaad ah ayaa la abuuri karaa iyadoo la adeegsanayo dibenzyl phosphoramite iyo tetrazolium, oo ku xiran koox hydroxyl ah, ka dibna oxidized si ay u sameeyaan koox fosfooryl ah deegaanka peroxyacid si loo dhamaystiro falcelinta.
Waqtiga post: Seb-08-2023