peptides-ku-beddelay methylation, oo sidoo kale loo yaqaanno peptides-ka methylation-la aqoonsan yahay, waa borotiinka qurxinta ka dambeeya tarjumaada (PTMs) waxayna ka ciyaaraan door muhiim ah oo nidaamin ah ku dhawaad dhammaan dhaqdhaqaaqyada nolosha ee unugyada.Borotiinnada waxaa katalyeeyay methyltransferase si loogu wareejiyo kooxaha hydroxyl hadhaaga amino acid ee gaarka ah si loogu xiro covalent.Methylation waa hannaan wax-ka-beddelid dib-u-noqosho ah oo ay kataliyed demethylases.
Daraasaduhu waxay muujiyeen in amino acids-ka caadiga ah ee methylated/demethylated ay badanaa yihiin lysine (Lys) iyo arginine (Arg).Daraasaduhu waxay ogaadeen in histone lysine methylation uu leeyahay hawlo kala duwan oo bayooloji ah sida ilaalinta unugyada asliga ah iyo qaybinta, firfircoonida X koromosoomyada, habaynta qoraalka iyo jawaabta dhaawaca DNA.", badiyaa waxay saamaysaa uumiga chromatin waxayna dib u dhigtaa muujinta hidda-wadaha."Histone arginine methylation wuxuu door muhiim ah ka ciyaaraa habaynta hiddo-wadaha qoraalka waxayna saameyn kartaa hababka kala duwan ee jireed ee unugyada, oo ay ku jiraan hagaajinta DNA-da, gudbinta calaamadaha, horumarinta unugyada, iyo kansarka.Sidaa darteed, Guopeptide Biology waxay si gaar ah u horumarisay tignoolajiyada peptides qurxinta methyl, kuwaas oo ay wax ka beddeleen saynisyahano ka dib turjumaadda borotiinka (PTMS) si ay uga caawiyaan cilmi-baarista.
Wax ka beddelka Methylation (Me1, Me2, Me3)
Fmoc-Lys (Me, Boc)-OH, Fmoc-Lys (Me2) -OH, Fmoc-Lys (Me3) -OH.HCL, Fmoc-Arg (Me, Pbf) -OH, Fmoc-Arg 2-OH.HCl (asymmetrical), F ayaa loo isticmaalay moc-Arg (me) 2-OH.HCl (symmetrical) iyo alaabta kale ee cayriin waxaa soo saaray FMOC habka adag ee wejiga si loo helo Lys iyo Arg methylated peptides, iyo alaabta waxaa sifeeyay HPLC.spectra mass oo khuseeya, HPLC chromatograms iyo COA ayaa la siiyay alaabta dhammaatay.
Waqtiga boostada: Nov-24-2023