peptides-ka unugyadu waa peptides yar yar oo si fudud u gali kara xuubka unugyada.Qaybtan molecules-ka ah, gaar ahaan CPP-yada leh hawlo la beegsanayo, waxay hayaan ballan qaadyo wax ku ool ah oo dawooyinka loo gaarsiinayo unugyada bartilmaameedka ah.
Sidaa darteed, cilmi-baarista ku saabsan waxay leedahay muhiimad nafleyda.Daraasaddan, CPP-yada leh waxqabadyo kala duwan oo transmembrane ah ayaa lagu bartay heerka isku xigxiga, iyagoo isku dayaya in ay ogaadaan arrimaha saameeya dhaqdhaqaaqa transmembrane ee CPP-yada, kala duwanaanshaha isku xigxiga ee u dhexeeya CPP-yada oo leh hawlo kala duwan iyo kuwa aan CPP-yada ahayn, oo soo bandhiga habka lagu falanqeynayo taxanaha noolaha.
CPP-yada iyo kuwa aan CPP-yada ahayn ayaa laga helay xogta CPPsite-ka iyo suugaanta kala duwan, iyo peptides transmembrane (HCPPs, MCPPs, LCPPs) oo leh waxqabad sare, dhexdhexaad, iyo hooseeya ayaa laga soo saaray taxanaha CPP-yada si loo dhiso xogta xogta.Iyadoo lagu salaynayo xogtan, daraasadaha soo socda ayaa la sameeyay:
1, Amino acid iyo qaabdhismeedka sare ee CPP-yada firfircoon ee kala duwan iyo kuwa aan CPP-yada ahayn ayaa lagu falanqeeyay ANOVA.Waxaa la ogaaday in isdhexgalka elektrostatic iyo hydrophobic ee acids amino ay door muhiim ah ka ciyaareen waxqabadka transmembrane ee CPPs, iyo qaab dhismeedka helical iyo gariiradda random ayaa sidoo kale saameeyay dhaqdhaqaaqa transmembrane ee CPPs.
2. Qalabka jireed iyo kiimikaad iyo dhererka CPP-yada oo leh hawlo kala duwan ayaa lagu soo bandhigay diyaaradda laba-geesoodka ah.Waxaa la ogaaday in CPP-yada iyo kuwa aan CPP-yada ahayn ee leh hawlo kala duwan lagu ururin karo qaar ka mid ah guryaha gaarka ah, iyo HCPP-yada, MCPP-yada, LCPP-yada iyo Aan-CPP-yada loo qaybiyay saddex kooxood, oo muujinaya kala duwanaanshahooda;
3. Warqaddan, waxaa la soo bandhigay fikradda centroid-ka jirka iyo kiimikaad ee isku xigxiga bayooloji, iyo hadhaaga ka kooban taxanaha waxa loo tixgaliyaa inay yihiin dhibco qaybeed, taxanahana waxa loo soo koobay hab-raac qaybeed cilmi-baadhiseed.Habkan ayaa lagu dabaqay falanqaynta CPP-yada iyadoo la saadaaliyay CPP-yo leh hawlo kala duwan oo lagu dul saaray diyaaradda 3D habka PCA-ga, waxaana la ogaaday in CPP-yada intooda badan ay isku urursan yihiin iyo qaar LCPPs ah oo la isku daray kuwa aan CPP-yada ahayn.
Daraasadani waxay saameyn ku leedahay naqshadeynta CPP-yada iyo fahamka kala duwanaanshaha taxanaha CPP-yada ee leh hawlo kala duwan.Intaa waxaa dheer, habka falanqaynta ee centroid jireed iyo kiimikaad ee taxanaha noolaha ee lagu soo bandhigay warqaddan ayaa sidoo kale loo isticmaali karaa falanqaynta dhibaatooyinka kale ee bayoolojiga.Isla mar ahaantaana, waxaa loo isticmaali karaa sidii jaangooyooyin gelineed ee qaar ka mid ah dhibaatooyinka kala soocida noolaha waxayna door ka ciyaaraan aqoonsiga qaabka.
Waqtiga boostada: Juun-15-2023