Fahamka alaabta bilawga ah ee daawada polypeptide

Daawooyinka Peptide guud ahaan waxaa lagu qeexaa inay yihiin polymers ka kooban amide bonds oo leh wax ka yar 40 hadhaaga amino acid.Sababtoo ah dhaqdhaqaaqa sare ee soo-dhoweynta iyo xulashada daawooyinka peptide ee leh khatarta hoose ee waxyeellooyinka, waxaa jiray xiiso xooggan oo loo qabo peptides ka warshadaha dawooyinka.Muddadan, waxa kale oo jiray dawooyin xiddigo badan, kuwaas oo inta badan ku urursanaa warshadaha cudurrada dheef-shiid kiimikaadka, sida GLP-1 analogue somalutide, gastric inhibitory peptide (GIP) glucagon-like peptide-1 (GLP-1) tesiparatide iyo dual kale. - agonists receptor.Intaa waxaa dheer, iyadoo kor u kaca daawooyinka PDC iyo RDC.Waqtigan xaadirka ah, hababka diyaarinta ee daawooyinka polypeptide inta badan waxaa ka mid ah isku dhafka kiimikada iyo halsano bayooloji.Biofermentation waxaa inta badan loo isticmaalaa si loo soo saaro peptides dheer.Faa'iidooyinka ayaa ah kharashyada wax-soo-saarka hooseeya, laakiin awood la'aanta in lagu soo bandhigo asiidhyada amino-ka aan caadiga ahayn ee isku xigxiga peptide iyo awood la'aanta in lagu sameeyo qurxinta kala duwan ee silsiladda peptide.Sidaa darteed, codsigeedu sidoo kale aad buu u xaddidan yahay.Hababka isku dhafka kiimikada waxaa ka mid ah isku-dhafka wajiga adag iyo isku-dhafka wajiga dareeraha.Isku-dubaridka wejiga adag wuxuu leeyahay faa'iido weyn marka loo eego isku-dhafka dareeraha-wejiga: maaddada xad-dhaafka ah ayaa loo isticmaali karaa fal-celinta si loo hubiyo isku-xirnaanta dhammaystiran.Asiidhyada amino-ka ee xad-dhaafka ah, wakiilada hoos u dhaca, iyo alaabada ayaa laga saari karaa hawlgallada nadiifinta fudud, ka fogaanshaha qallafsanaanta ka dib-socodka iyo hawlgallada sifaynta iyo hagaajinta waxtarka shaqada, markaa habka isku-dhafka adag ee adag ayaa ahaa midka ugu ballaaran ee loo isticmaalo."Waxyaabaha ceeriin ee kiimikada ah ee isku dhafka peptides waxaa ka mid ah alaabta bilawga ah, reagents, iyo dareerayaasha."Tayadooda, gaar ahaan tayada alaabta bilowga ah, waxay ku yeelan kartaa saameyn kala duwan tayada API-ga.Maaddada bilawga ah waxay inta badan tixraacaysaa derajooyinka amino acid ee dammaanadda leh ee silsiladda peptide ee la beddelay asiidh dufan ah, polyetylen glycol, iwmSidaa darteed, waa inaan diirada saarnaa xakamaynta alaabta bilawga ah.

I. Qiimee xulashada alaabta bilowga ah

ICHQ11 waxay si cad u soo jeedinaysaa in haddii badeecada kiimikaad ee lagu iibiyo suuqa loo isticmaalo alaab bilaw ah, codsaduhu caadi ahaan uma baahna inuu ka hadlo macquulnimadeeda.Alaabooyinka kiimikaad ee lagu iibiyo suuqa guud ahaan looma isticmaali karo oo kaliya sida agabka bilawga ah ee dawooyinka, laakiin sidoo kale waxa lagu iibin karaa suuqyada aan dawooyinka ahayn.Iskudhisyada la habeeyey iyo kuwa la farsameeyey kama mid aha alaabada kiimikaad ee lagu iibiyo suuqa.Inkasta oo aanu jirin suuq aan dawo ahayn oo lagu ilaaliyo amino acids si ay u buuxiso qeexida ICHQ11 ee kiimikooyinka lagu iibiyo suuqa, waa kuwo is haysta, kimikal ahaan kala duwan oo qaab dhismeedkoodu cad yahay, fududahay in la go'doomiyo lana sifeeyo, waxaana lagu aqoonsan karaa laguna tijaabin karaa hababka falanqaynta guud. .Waxay leeyihiin sifooyin kiimikaad oo deggan wayna fududahay in la kaydiyo, la raro, lana sameeyo

Ii.Xakamaynta walxaha laxiriira ee ku jira walxaha bilawga ah

Asiidhyada amino-ka ilaalinta ee aan soo sheegnay ayaa lagu dhex daray qaab dhismeedka API oo ah qayb dhismeed oo muhiim ah, kaas oo si toos ah ula xidhiidha tayada API-ga.Sidaa darteed, waa in aan si adag u xakameyno nuxurka wasakhda ah ee ku jira alaabta bilowga ah, fahamsanahay isbeddelka iyo ka saarista wasakhdaas habka la aasaasay, oo ugu dambeyntii caddeeyo xiriirka ka dhexeeya iyaga iyo wasakhda API.

Fahamka alaabta bilawga ah ee daawada polypeptide

Seddexaad, haraaga dareeraha ee walxaha bilowga ah

Guud ahaan, marka la eego gaar ahaaneed ee jiilka adag ee peptides, xaddi badan oo dareere ah ayaa loo isticmaali doonaa si loo nadiifiyo resin peptide ka dib marka la dhammeeyo tallaabo kasta oo isku xidhka amino acid iyo ka-goynta ilaalinta.peptides-ka cayriin ee lagu helo dildilaaca resin-ka peptide sidoo kale waxa samayn doona HPLC oo la qaboojiyey.Markaa, waxaa jirta khatar yar oo ah in qadarka yar ee dareeraha ah ee ku xidhan asiidhyada amino-ka difaaca la geeyo API-ga kama dambaysta ah.Si kastaba ha ahaatee, fiiro gaar ah waa in la siiyaa hadhaaga acetate, butyl acetate, iyo dareerayaasha khamriga, maadaama dareerayaashani ay sababi karaan waxyeelooyin leh asiidhyada amino-ka firfircoon ama silsiladaha peptide inta lagu jiro isku dhafka firfircoon ee amino acids.Tusaale ahaan, inta lagu guda jiro isku-xidhka amino acid, acetic acid-ka haraaga ah ayaa ka falcelin doona kooxda amino ee daboolka ah ee silsiladda peptide, taas oo keeneysa dhammaadka xiran ee silsiladda peptide;Inta lagu jiro hawlaha amino acid, dareeraha aalkolada ee haraaga ah ayaa laga yaabaa inay la falgalaan kooxda karboksyl ee firfircoon, taas oo horseedaysa dhaqdhaqaaqa amino acid firfircoon, hoos u dhigista u dhigma amino acid, ugu dambeyntiina waxay keenaysaa isku-xidhka amino acid-ka oo aan dhamaystirnayn iyo la'aanta wasakhda peptide.Shirkaddu waxay maamushaa butyl acetate, aalkolada, methanol, iyo acetic acid gudaha COA, iyadoo tusaale ahaan ka qaadanaysa amino acid Zheng Yuan Biochemical.Heerka butyl acetate wuxuu ahaa ≤0.5% butyl acetate, kaas oo dhab ahaantii la ogaaday inuu yahay 0.10%.Marka loo eego ICHQ3C, butyl acetate ee saddexda nooc ee dareeraha, waxay dejiyaan heerka 0.5% ama ka yar ee waafaqsan shuruudaha ICHQ3C, laakiin tixgelinta butyl acetate amino acetylation waxay horseedi kartaa khatarta, sidoo kale waxay la macaamilaan butyl acetate si loo habeeyo cilmi-baarista , si loo go'aamiyo heerar ku habboon.