Waxaa jira noocyo badan oo peptides transmembrane ah, kala soociddooduna waxay ku salaysan tahay sifooyinka jirka iyo kiimikaad, ilaha, hababka la dhuuqo, iyo codsiyada biomedical.Marka loo eego sifooyinka jidheed iyo kiimikaad, xuubka peptides ee galaya waxa loo qaybin karaa saddex nooc: cationic, amphiphilic iyo hydrophobic.Xuubka xuubka xuubka ee xuubka xuubka ee loo yaqaan 'peptides' wuxuu xisaabiyaa 85%, halka xuubka hydrophobic ee gala peptides ay ka yihiin kaliya 15%.
1. Xuubka xuubka ee peptide gasha
Cationic transmembrane peptides waxay ka kooban yihiin peptides gaaban oo hodan ku ah arginine, lysine, iyo histidine, sida TAT, Penetratin, Polyarginine, P22N, DPV3 iyo DPV6.Waxaa ka mid ah, arginine waxa ku jira guanidine, kaas oo hydrogen ku xidhi kara kooxaha phosphoric acid ee si xun loogu soo oogay xuubka unugyada iyo dhexdhexaadinta peptides transmembrane ee xuubka hoos yimaada xaaladda PH-physiological qiimaha.Daraasadaha oligarginine (laga bilaabo 3 R ilaa 12 R) waxay muujiyeen in awoodda dhexgalka xuubka la helay kaliya marka qadarka arginine uu hooseeyo 8, iyo awoodda xuubka xuubka ayaa si tartiib tartiib ah u kordhay kororka qadarka arginine.Lysine, inkasta oo cationic sida arginine, kuma jiraan guanidine, marka marka ay keligeed jirto, waxtarka xuubka xuubka ma aha mid aad u sarreeya.Futaki iyo al.(2001) waxay ogaatay in saamaynta dhexgalka xuubka wanaagsan la gaari karo kaliya marka xuubka unugga cationic ee galaya peptide uu ka kooban yahay ugu yaraan 8 amino acids oo si togan loo dallaco.In kasta oo hadhaaga amino acid-ka si togan loo dallaco ay muhiim u yihiin peptides-ka dhex geli kara xuubka, haddana asiidhyada amino-yada kale ayaa si isku mid ah muhiim u ah, sida marka W14 ay isu beddesho F, penetrability ee Penetratin ayaa lumaya.
Qayb gaar ah oo ka mid ah peptides transmembrane cationic waa taxanaha nukliyeerka nukliyeerka (NLSs), kaas oo ka kooban peptides gaaban oo hodan ku ah arginine, lysine iyo proline waxaana loo qaadi karaa nukleus iyada oo loo marayo isku-dhafka daloolka nukliyeerka.NLS-yada waxa loo sii kala qaybin karaa hal iyo laba teeb, oo ka kooban hal iyo laba rucubood oo asiidhyada amino ee aasaasiga ah, siday u kala horreeyaan.Tusaale ahaan, PKKRKV ee ka yimid fayraska simian 40(SV40) waa hal wax oo NLS ah, halka borotiinka nukliyeerka uu yahay laba jeer NLS.KRPAATKKAGQAKKL waa isku xigxiga gaaban ee door ka ciyaari kara xuub-wareenka.Sababtoo ah NLS-yada intooda badani waxay leeyihiin nambaro lacag ah oo ka yar 8, NLS-yadu maahan peptides transmembrane wax ku ool ah, laakiin waxay noqon karaan peptides transmembrane oo waxtar leh marka si wadajir ah loogu xiro taxanaha peptide hydrophobic si ay u sameeyaan amphiphilic transmembrane peptides.
2. Amphiphilic transmembrane peptide
Amphiphilic transmembrane peptides waxay ka kooban yihiin hydrophilic iyo hydrophobic domains, kuwaas oo loo qaybin karo amphiphilic hoose, secondary α-helical amphiphilic, β-folding amphiphilic iyo prolin-enriched amphiphilic.
Nooca aasaasiga ah ee loo yaqaan 'amphiphilic wear membrane peptides' oo loo qaybiyo laba qaybood, qayb leh NLSs oo si wadajir ah ugu xiran isku xigxiga hydrophobic peptide, sida MPG (GLAFLGFLGAAGSTMGAWSQPKKKRKV) iyo Pep - 1 (KETWWETWWTEWSQPKKRKV), Labaduba waxay ku saleysan yihiin calaamada meelaynta nukliyeerka PKKKRKV ee SV4 domain MPG waxa uu la xidhiidha isku dhafka isku dhafka ah ee HIV glycoprotein 41 (GALFLGFLGAAGSTMG A), iyo qaybta hydrophobic ee Pep-1 waxay la xiriirtaa cluster hodanka ah tryptophan oo leh xirnaansho xuub sare leh (KETWWET WWTEW).Si kastaba ha ahaatee, aagagga hydrophobic ee labadaba waxay ku xiran yihiin calaamadaynta meelaynta nukliyeerka PKKKRKV iyada oo loo marayo WSQP.Fasal kale oo ah peptides transmembrane amphiphilic aasaasiga ah ayaa laga soocay borotiinnada dabiiciga ah, sida pVEC, ARF (1-22) iyo BPrPr (1-28).
Qaybta labaad ee α-helical amphiphilic transmembrane peptides waxay ku xidhmaan xuubka α-helices, iyo hadhaaga hydrophilic iyo hydrophobic amino acid waxay ku yaalaan sagxadaha kala duwan ee qaab dhismeedka helical, sida MAP (KLALKLAK ALKAALKLA).Isku laablaabashada beta peptide nooca amphiphilic wear xuubka, awooda ay u leedahay samaynta xaashida xuubka beta waxay muhiim u tahay awoodeeda galitaanka xuubka, sida VT5 (DPKGDPKGVTVTVTVTVTGKGDPKPD) ee habka cilmi baarista awooda galitaanka xuubka, iyadoo la adeegsanayo nooca D Analoogyada isu beddelka amino acid ma samayn karaan gabal laalaabmay beta, awoodda dhexgalka xuubku aad bay u liidataa.In proline-enriched amphiphilic transmembrane peptides, polyproline II (PPII) si fudud ayaa loo sameeyay biyo saafi ah marka proline si aad ah loo kobciyo qaab dhismeedka polypeptide.PPII waa helix bidix-bidix leh oo leh 3.0 hadhaaga amino acid halkii marba, taas oo ka soo horjeeda qaabka caadiga ah ee alfa-helix ee gacanta midig oo leh 3.6 hadhaaga amino acid halkii marba.Proline-enriched amphiphilic transmembrane peptides waxaa ka mid ah peptide antimicrobial bovine 7 (Bac7), polypeptide synthetic (PPR) n (n waxay noqon kartaa 3, 4, 5 iyo 6), iwm.
3. Xuubka hydrophobic ee gala peptide
Hydrophobic transmembrane peptides waxaa ku jira oo kaliya hadhaaga amino acid-ka aan polar-ka ahayn, oo leh lacag saafi ah oo ka yar 20% wadarta kharashka isku xigxiga amino acid, ama waxa ku jira unugyo hydrophobic ama kooxo kiimiko ah oo lagama maarmaan u ah transmembrane.Inkasta oo peptides-ka transmembrane-ka unugyada inta badan la iska indho-tiro, way jiraan, sida koritaanka fibroblast factor (K-FGF) iyo koritaanka fibroblast factor 12 (F-GF12) ee Kaposi's sarcoma.
Waqtiga boostada: Mar-19-2023