Habka soo saarista
Sanadihii 1950-meeyadii iyo 1960-meeyadii, dalal badan oo adduunka ah, oo uu ku jiro Shiinaha, ayaa inta badan laga soo saaray peptides xubnaha xayawaanka.Tusaale ahaan, cirbadeynta thymosin waxaa lagu diyaariyaa in la gowraco dibi dhallan, oo laga saaro thymus, ka dibna la isticmaalo oscillating separation biotechnology si loo kala saaro peptides iyo kubka thymus.Thymosin-kan waxa si weyn loogu isticmaalaa habaynta iyo kor u qaadida shaqada difaaca unugga ee bini aadamka.
Peptides bioactive dabiiciga ah ayaa si ballaaran loo qaybiyaa.Waxaa jira peptides bioactive badan oo ku jira xayawaanka, dhirta iyo noolaha badda ee dabiiciga ah, kuwaas oo ciyaara hawlo nafsiyeed oo kala duwan oo ilaaliya hawlaha nolosha caadiga ah.peptides-ka dabiiciga ah ee dabiiciga ah waxaa ka mid ah dheef-shiid kiimikaad sare ee noolaha sida antibiyootiga iyo hormoonnada, iyo sidoo kale peptides bioactive ee ku jira hababka kala duwan ee unugyada.
Waqtigan xaadirka ah, peptides badan oo bioactive ah ayaa laga soocay aadanaha, xayawaanka, dhirta, microbial iyo noolayaasha badda.Si kastaba ha ahaatee, peptides bioactive ayaa guud ahaan laga helaa xaddi hoose oo noolaha, iyo farsamooyinka hadda jira ee go'doominta iyo nadiifinta peptides bioactive ee noolaha dabiiciga ah ma ahan kuwo qumman, oo leh qiimo sare iyo bioactivity hooseeya.
Hababka caadiga ah ee loo isticmaalo soo saarista peptide iyo kala-soocidda waxaa ka mid ah milix, ultrafiltration, shaandhaynta jel, roobka dhibicda isoelectric, chromatography sarrifka ion, chromatography affinity, chromatography adsorption, gel electrophoresis, iwm. Khasaaraha ugu weyni waa kakanaanta hawlgalka iyo qiimaha sare.
Habka Acid-saldhigga
Acid iyo alkali hydrolysis inta badan waxaa lagu isticmaalaa machadyada tijaabada ah, laakiin marar dhif ah ayaa loo adeegsadaa waxqabadka wax soo saarka.Habka hydrolysis alkaline ee borotiinka, inta badan asiidhyada amino sida serine iyo threonine waa la burburiyaa, jinsiyadu waxay dhacdaa, iyo tiro badan oo nafaqooyin ah ayaa lumay.Sidaa darteed, habkan si dhif ah ayaa loo isticmaalaa wax soo saarka.Acid hydrolysis ee borotiinada ma keento jinsiyada amino acids, hydrolysis waa degdeg ah iyo falcelinta waa dhamaystiran.Si kastaba ha ahaatee, khasaaraheedu waa tignoolajiyada adag, xakamaynta adag iyo wasakhowga deegaanka ee halista ah.Qaybinta miisaanka kelli ee peptides waa mid aan sinnayn oo aan degganayn, iyo hawlahooda jireed way adagtahay in la go'aamiyo.
Hydrolysis enzymatic
Inta badan peptides bioactive waxaa laga helaa silsilado dhaadheer oo borotiinno ah oo ku jira xaalad aan firfircoonayn.Marka hydrolyzed by protease gaar ah, peptide kooda firfircoon ayaa laga sii daayaa taxanaha amino ee borotiinka.Soo saarista enzymatic ee peptides-ka noolaha ee xayawaanka, dhirta iyo noolaha badda ayaa ahaa cilmi-baaris diiradda lagu saaray tobannaankii sano ee la soo dhaafay.
Hydrolysis Enzymatic ee peptides bioactive waa xulashada borotiinada ku habboon, iyada oo la adeegsanayo borotiinno sida substrates iyo borotiinada hydrolyzing si loo helo tiro badan oo peptides bioactive ah oo leh hawlo jireed oo kala duwan.Nidaamka wax soo saarka, heerkulka, qiimaha PH, xoojinta enzyme, xoojinta substrate-ka iyo arrimo kale ayaa si dhow ula xiriira saameynta enzymatic hydrolysis ee peptides yaryar, furahana waa doorashada enzyme.Sababtoo ah enzymes kala duwan oo loo isticmaalo hydrolysis enzymatic, xulashada iyo samaynta enzymes, iyo ilaha borotiinka ee kala duwan, peptides-ka soo baxa ayaa si aad ah ugu kala duwan miisaanka, qaybinta miisaanka kelli, iyo halabuurka amino acid.Caadi ahaan qofku wuxuu doortaa borotiinka xayawaanka, sida pepsin iyo trypsin, iyo borotiinada dhirta, sida bromelain iyo papain.Iyadoo horumarinta sayniska iyo tignoolajiyada iyo hal-abuurnimada joogtada ah ee tignoolajiyada ensaymka noolaha, waxaa la ogaan doonaa oo la isticmaali doonaa enzymes aad u badan.Hydrolysis Enzymatic ayaa si weyn loogu isticmaalay diyaarinta peptides bioactive sababtoo ah tignoolajiyada qaan-gaarka ah iyo maalgashiga hooseeya.
Waqtiga boostada: Meey-30-2023