Sida loo farsameeyo CGD cyclopeptide

Integrin, ama integrin, waa heterodimer transmembrane glycoprotein reseptor kaas oo dhexdhexaadiya ku dheggan unugyada xayawaanka iyo calaamadaynta.Waxay ka kooban tahayα iyoβ qayb hoosaadyo.Waxay ku lug leedahay hagaajinta ficilada gacanta ee kala duwan oo ay ku jiraan guuritaanka unugyada, dhexgalka unugyada, unugyada iyo calaamadaha intercellular, adhesion cell, iyo hababka angiogenesis.Integrinαvβ3 ayaa hadda si ballaaran loo sahamiyay.Muuqaalka integrinαvβ3 waxay si dhow ula xiriirtaa guuritaanka burada, angiogenesis, caabuqa iyo lafo-jileeca.Integrin ayaa si aad ah loogu muujiyay dhammaan unugyada burooyinka iyo xuubabka unugyada endothelial ee neovascularization.Muuqashada integrin waxay si dhow ula xiriirtaa guuritaanka burada iyo angiogenesis.Sanadihii la soo dhaafay, daraasaadka sayniska ayaa muujiyay in ay jiraan 11 integrins kuwaas oo si gaar ah ugu xidhi kara RGD peptide, kuwaas oo ah peptides ka soo horjeeda soo-qabayaasha integrin.

RGD peptide waxaa loo kala saaray RGD peptide toosan iyo RGD peptide wareegtada.Marka la barbar dhigo RGD peptide toosan, RGD peptide cyclic peptide waxa uu leeyahay waafaqid soo dhawayn xoog leh iyo gaar ahaanshaha soo dhawaynta.Kuwa soo socdaa waa noocyada caadiga ah iyo hababka isku dhafan ee peptide wareegtada RGD.

Noocyada caadiga ah ee RGD peptides meerto ah:

1. peptides meerto ah oo ka kooban taxane RGD oo ay sameeyeen curaarta disulfide

2. peptides meerto ah oo ka kooban taxanaha RGD ee ay sameeyeen curaarta amide

Isku dhafka RGD peptide meertada ah:

Qaabka utility waxa uu la xidhiidhaa habka isku xidhka peptide meerto ee RGD ee goobta farsamada adag ee polypeptide synthesis.Habka cusub ayaa ah in la doorto 2-chloro-triphenylmethyl chloride resin sida gudbiyaha shardiga ah, marka hore ku xidh kooxda silsiladda carboxyl ee ugu horeysa koox gaar ah oo ilaalinaysa D aspartic acid amino acid, ka dibna ku xidh peptide toosan ee peptide isku xigxiga RGD , iyo amino acid kii ugu dambeeyay ee meesha ka saaray kooxda difaaca ee FMOC oo aan lahayn piperidine.Dareeraha la cayimay ayaa lagu daray si looga saaro silsiladda dhinaca ee kaarboxyl kooxda difaaca ee D aspartic acid ee ugu horreeya si toos ah xabagta, oo ay ku xigto ku darida piperidine si ay uga saarto kooxda difaaca amino FMOC ee dhamaadka amino acid, oo ay ku xigto ku darida wakiilka isku xiran. si loo fuuqbaxo oo loo ururiyo kooxda kaarboxyl iyo kooxda amino ee ka soo ifbaxday madaxa iyo dhammaadka peptide-ka tooska ah ee tooska ah ee si toos ah uga soo baxa resin ee qaabka amide bond si loo soo saaro peptide wareeg ah.Ugu dambeyntii, peptide-ka wareegtada ah ayaa si toos ah looga gooyaa resinka oo leh xalka goynta.