Marka loo eego habka isku xirka amino acid iyo sonkorta, peptide sonkorta waxaa loo qaybin karaa afar qaybood: O glycosylation, C a N glycosylation, saccharification dew iyo GPI (glycophosphatidlyinositol).
1. N-glycosylation glycopeptides waxay ka kooban yihiin gulukooska N-acetamide dhamaadka dhimista silsiladda glycan (Glc-Nac) ee ku xiran N atamka ee kooxda amide ee silsiladda dhinaca ee Asn ee silsiladda peptide, iyo Asn Awood u leh isku xirka silsiladda glycan waa in ay ku taal AsN-X-Ser /Thr (X! = P) qaabka ay sameeyeen hadhaaga.Sonkorta waa N-acetylglucosamine.
N-glycosylation glycopeptide qaab dhismeedka wax laga beddelay
2. Qaab dhismeedka O-glycosylation wuu ka fudud yahay N-glycosylation.Glycopeptide-kan guud ahaan wuu ka gaaban yahay glycan, laakiin wuxuu leeyahay noocyo ka badan N-glycosylation.Ser iyo Thr guud ahaan waa glycosylated ee silsiladda peptide.Intaa waxaa dheer, waxaa jira glycopeptides lagu qurxiyey tyrosine, hydroxyl, iyo hydroxyproline glycosylation.Booska isku xirka waa atamka oksijiinta hydroxyl ee silsiladda dhinaca ee hadhaaga.Sonkorta isku xidhan waa galactose ama N-acetylgalactosamine (Gal&GalNAc) ama glucose/glucosamine (Glc/GlcNAc), mannose/mannosamine (Man/ManNAc), iwm.
O-glycosylation waxay wax ka beddeshaa qaabka
3. Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine/acetylglucosamine)
Hal N-acetylglucosamine (GlcNAc) glycosylation waxay ku xidhaa borotiinada O-GlcNAc atamka oksijiinta hydroxyl ee serine ama hadhaaga threonine ee borotiinka.O-GlcNA glycosylation waa O-GlcNAc monosaccharide qurxinta iyada oo aan la kordhin glycan;Sida fosforyaalka peptide, O-GlcNAc glycosylation of glycopeptides sidoo kale waa habka qurxinta borotiinka firfircoon.Qurxinta O-GlcNAc ee aan caadiga ahayn waxay sababi kartaa cudurro kala duwan sida sonkorowga, cudurada wadnaha, burooyinka, cudurka Alzheimers iyo wixii la mid ah.
Dhibcaha glycosylation ee glycopeptides
Qaab dhismeedka aasaasiga ah ee polypeptide iyo silsiladaha sonkorta waxay ku xidhan yihiin silsiladaha borotiinka ee curaarta covalent, iyo goobaha isku xidha silsiladaha sonkorta waxa loo yaqaan goobaha glycosylation.Maaddaama aysan jirin template si loo raaco biosynthesis ee silsiladaha sonkorta glycopeptide, silsilado sonkor ah oo kala duwan ayaa lagu dhejin doonaa isla goobta glycosylation, taasoo keentay waxa loogu yeero inhomogeneity microscopic.
Glycosylation ee glycopeptides
1. Saamaynta glycopeptide glycosylation ee daawaynta-wax ku oolnimada borotiinnada daweynta
Marka laga hadlayo borotiinka-daaweynta, glycosylation waxay sidoo kale saamaysaa nolosha nuska ah iyo bartilmaameedka daawooyinka borotiinka ee vivo
2. Glycopeptide glycosylation iyo borotiinada milmay
Daraasaduhu waxay muujiyeen in silsiladaha sonkorta ee dusha sare ee borotiinnada ay hagaajin karaan milanka molecular ee borotiinnada
3. Glycopeptide glycosylation iyo difaaca borotiinka
Dhinaca kale, silsiladaha sonkorta ee dusha sare ee borotiinada waxay keeni karaan jawaab celin difaac oo gaar ah.Dhanka kale, silsiladaha sonkorta waxay dabooli karaan sagxadaha qaar ee dusha borotiinka waxayna yareeyaan difaacooda
4. Glycopeptide glycosylation oo kordhisa xasilloonida borotiinka
Glycosylation waxay kordhin kartaa xasilloonida borotiinnada xaaladaha kala duwan ee denaturation (sida denaturants, kulaylka, iwm.) kana fogaato isku-darka borotiinnada.Isla mar ahaantaana, silsiladaha sonkorta ee dusha sare ee borotiinku waxay sidoo kale dabooli karaan qaar ka mid ah dhibcaha hoos u dhaca borotiinka ee molecules borotiinka, taas oo kordhinaysa iska caabinta borotiinka borotiinka.
5. Glycopeptide glycosylation kaas oo saameeya dhaqdhaqaaqa noolaha ee molecules borotiinka
Beddelidda glycosylation borotiinku waxay sidoo kale awood u siin kartaa molecules borotiinka inay sameeyaan dhaqdhaqaaqyo noole oo cusub
Waqtiga boostada: Agoosto-03-2023