Nidaamka naqshadeynta iyo xalka silsiladda peptide polypeptide

I. Soo koobid
Peptides waa macromolecules gaar ah oo ay ka mid yihiin taxane aan caadi ahayn marka la eego sifooyinkooda kiimikaad iyo kuwa jireed.Qaar ka mid ah peptides way adag tahay in la farsameeyo, halka kuwa kalena ay fudud yihiin in la farsameeyo laakiin ay adag tahay in la sifeeyo.Dhibaatada la taaban karo ayaa ah in peptides intooda badani ay wax yar ku milmayaan xalalka aqueous, sidaas darteed nadiifintayada, qaybta u dhiganta peptide hydrophobic waa in lagu milmaa dareerayaasha aan biyaha lahayn, Sidaa darteed, kuwan dareeraha ah ama baqshiishyada waxay u badan tahay inay si aad ah u khilaafsan yihiin isticmaalka hababka tijaabada bayoolojiga, si farsamayaqaannada si adag looga mamnuuco inay u isticmaalaan peptide ujeeddooyinkooda, si ay kuwan soo socdaa u yihiin dhowr qaybood oo naqshadeynta peptides ee cilmi-baarayaasha.

Nidaamka naqshadeynta iyo xalka silsiladda peptide polypeptide
Marka labaad, doorashada saxda ah ee peptides adag synthetic
1. Wadarta dhererka isku xigxiga hoos loo habeeyey
Peptides oo ka yar 15 hadhaa waa ay fududahay in la helo sababtoo ah xajmiga peptide-ku wuu kordhaa iyo nadiifnimada alaabta ceyriinka ah ayaa hoos u dhacda.Maaddaama dhererka guud ee silsiladda peptide-ku uu kordho in ka badan 20 hadhaaga ah, tirada saxda ah ee alaabta ayaa ah welwel muhiim ah.Tijaabooyin badan, way fududahay in la helo saameyn lama filaan ah adiga oo hoos u dhigaya lambarka haraaga ee ka hooseeya 20.
2. Hoos u dhig tirada haraaga biyo-cabbiyeedka
Peptides oo ay ku badan yihiin haraaga hydrophobic, gaar ahaan gobolka 7-12 hadhaaga C-terminus, waxay badanaa keenaan dhibaatooyin synthetic.Tan waxaa loo arkaa isku darka aan ku filneyn si sax ah sababtoo ah xaashi B-laab ah ayaa laga helay isku-dhafka."Xaaladahan oo kale, waxaa laga yaabaa inay faa'iido u leedahay in la beddelo wax ka badan laba haraaga xun iyo kuwa taban, ama in Gly ama Pro la geliyo peptide si loo furo halabuurka peptide."
3. Hoos u dhigista hadhaaga "adag".
"Waxaa jira tiro ka mid ah Cys, Met, Arg, iyo Isku day hadhaaga kuwaas oo aan guud ahaan si sahal ah loo soo saarin."Ser waxaa caadi ahaan loo isticmaali doonaa beddelka aan oksaydhiyaha ahayn ee Cys.
Nidaamka naqshadeynta iyo xalka silsiladda peptide polypeptide

Saddexaad, wanaaji doorashada saxda ah ee lagu milmi karo biyaha
1. Hagaajin meesha N ama C
Marka loo eego peptides acidic (taas oo ah, si xun loogu dallacay pH 7), acetylation (N-terminus acetylation, C terminus had iyo jeer ilaalinta koox karboxyl ah oo bilaash ah) ayaa si gaar ah lagula talinayaa in la kordhiyo kharashka taban.Si kastaba ha noqotee, peptides aasaasiga ah (taas oo ah, si togan loogu dallaco pH 7), amine (koox amino bilaash ah oo ku yaal N-terminus iyo amination at C-terminus) ayaa si gaar ah lagula talinayaa in la kordhiyo kharashka togan.

2. Si weyn u gaabin ama u dheeree isku xigxiga

Qaar ka mid ah taxanaha waxa ku jira tiro badan oo ah amino acids hydrophobic, sida Trp, Phe, Val, Ile, Leu, Met, Tyr iyo Ala, iwm.Waxaa laga yaabaa inay faa'iido leedahay in la dheereeyo isku xigxiga si loo sii kordhiyo tirarka togan iyo kuwa taban ee peptide.Doorashada labaad waa in la yareeyo xajmiga silsiladda peptide si loo kordhiyo tiirarka togan iyo kuwa taban iyadoo hoos loo dhigayo hadhaaga hydrophobic.Marka ay sii xoogaystaan ​​dhinacyada togan iyo kuwa taban ee silsiladda peptide, waxay u badan tahay inay ku falceliso biyaha.
3. Ku rid hadhaa biyo-milmi leh
Qaar ka mid ah silsiladaha peptide, isku darka qaar ka mid ah acids amino togan iyo kuwa taban waxay hagaajin karaan milanka biyaha.Shirkaddayadu waxay ku talinaysaa N-terminus ama C-terminus ee peptides acidic in lagu daro Glu-Glu.N ama C terminus ee peptide aasaasiga ah ayaa la siiyay ka dibna Lys-Lys.Haddii kooxda la soo dacweeyay aan la meeleyn karin, Ser-Gly-Ser sidoo kale waxaa lagu dhejin karaa N ama C terminus.Si kastaba ha ahaatee, habkani ma shaqeynayo marka dhinacyada silsiladda peptide aan la bedeli karin.